Lately there has been a lot of discussion among insiders and those closely following the COVID "mRNA vaccine" story concerning contamination of the mRNA vaccines with DNA fragments which include DNA sequences derived from Simian Virus 40.
Background: In vitro transcription reactions used to generate nucleoside modified RNA for SARS-CoV-2 vaccines currently rely on an RNA polymerase transcribing from a DNA template.
Recently, DNA sequencing studies have revealed this plasmid DNA at significant levels in both Pfizer-BioNTech and Moderna modRNA vaccines.
Conclusion: These data demonstrate the presence of billions to hundreds of billions of DNA molecules per dose in these vaccines.
Using fluorometry, all vaccines exceed the guidelines for residual DNA set by FDA and WHO of 10 ng/dose by 188 - 509-fold.
QPCR residual DNA content in all vaccines were below these guidelines emphasizing the importance of methodological clarity and consistency when interpreting quantitative guidelines.
If you want to manufacture large amounts of pure RNA, you basically need to start with large amounts of DNA, and use a protein enzyme plus RNA chemical subunits and a source of energy to make RNA from the DNA. Then you need to break down the DNA into small fragments while still leaving the larger RNA intact.
https://brownstone.org/articles/what-is-adulteration-of-vaccines-and-why-should-you-care/
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