The Wuhan Institute of Virology assembled a monkeypox virus genome, allowing the virus to be identified through PCR tests, using a method researchers flagged for potentially creating a "Contagious pathogen," The National Pulse can reveal.
Researchers appeared to identify a portion of the monkeypox virus genome, enabling PCR tests to identify the virus, in the paper: "Efficient Assembly of a Large Fragment of Monkeypox Virus Genome as a qPCR Template Using Dual-Selection Based Transformation-Associated Recombination."
Monkey pox viruses - referred to as "MPXVs" in the paper - have strains that are "More pathogenic and [have] been reported to infect humans in various parts of the world."
"Since MPXV infection has never been associated with an outbreak in China, the viral genomic material required for qPCR detection is unavailable. In this report, we employed dual-selective TAR to assemble a 55-kb MPXV genomic fragment that encompasses E9L and C3L, two valuable qPCR targets for detecting MPXV or other orthopoxviruses."
"The primary purpose of assembling a fragment of the MPXV genome is to provide a nucleotide template for MPXV detection," reiterated the study, which relied on the process of transformation-associated recombination to isolate a genomic fragment of the monkeypox virus.
The paper acknowledged that TAR "Applied in virological research could also raise potential security concerns, especially when the assembled product contains a full set of genetic material that can be recovered into a contagious pathogen."
The unearthed study follows the Wuhan Institue of Virology conducting similar research into strains of bat coronaviruses that could infect humans while admitting its facilities lacked proper laboratory safety protocols.
https://thenationalpulse.com/2022/05/22/wuhan-assembled-monkeypox-strains/
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